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Carl Zeiss Microscopy GmbH) with a 40 objective lens and ZEN 2011 v14 (Carl Zeiss)

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The samples were diluted to concentrations between 600 and 1400 g/mL in 50% methanol

Moreover, in studying the course of acute liver failure caused by the hepatitis E virus, ASS1 was pronouncedly elevated in serum-derived EVs and closely correlated with the progression of liver failure, which may implicate a disturbance of cellular arginine metabolism [187]

4-micron sections were stained with hematoxylin and eosin (H&E)